Best stacking method for protozoa

Optical microscopes + digital photography

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MikeCT
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Joined: 08.04.2014 19:56

Best stacking method for protozoa

Unread postby MikeCT » 13.08.2014 13:21

I am photography protozoa, which are basically transparent, and the resultant stacks are not as clear as I would like. Do any of you have experience with this, and do you haste any advice?

Many thanks

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Stas Yatsenko
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Re: Best stacking method for protozoa

Unread postby Stas Yatsenko » 14.08.2014 10:24

I can only suggest to use method C, as it should deal with semitransparent structures better than other methods.

MikeCT
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Joined: 08.04.2014 19:56

Re: Best stacking method for protozoa

Unread postby MikeCT » 20.08.2014 16:32

Thanks: interestingly each of the three methods is better in some parts of the image than others. However, Method C does seem to be the overall winner.

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Stas Yatsenko
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Re: Best stacking method for protozoa

Unread postby Stas Yatsenko » 22.08.2014 11:50

Thanks. You can process the stack with different methods and then combine results on the Retouching tab. New version allows you to use alternative results as a source for cloning.


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