Artifacts on "spikes" projecting from diatom
Posted: 12.08.2011 01:38
Hello, I have been experimenting with stacking images of diatoms. I recently acquired a stepper motor so I could try out deeper stacks and see if I could produce good visualizations of "thicker" specimens. I chose the attached specimen because it has a lot of "depth" to it. The main part of the frustule has come out modestly well in this stack. However, the spikes projecting nearly vertically from the surface have significant stacking artifacts. I was wondering if anyone could suggest what I might do to minimize these artifacts.
If you look at the spikes you can see that the spikes have significant "waves" in them. If you consider the frustule as the face of a clock, the problem is more severe between 1-o'clock and 5-o'clock. I freely admit that this is a challenging specimen. The relief of the main body of the frustule changes more slowly than the spikes, but there are a fairly large number of images which "slice" the spike region in the source stack. I have tried to adjust the radius and smoothing parameters. Reducing the radius from the default significantly degrades the image. Changing the smoothly does not appear to help either.
Thanks in advance,
Bryan
If you look at the spikes you can see that the spikes have significant "waves" in them. If you consider the frustule as the face of a clock, the problem is more severe between 1-o'clock and 5-o'clock. I freely admit that this is a challenging specimen. The relief of the main body of the frustule changes more slowly than the spikes, but there are a fairly large number of images which "slice" the spike region in the source stack. I have tried to adjust the radius and smoothing parameters. Reducing the radius from the default significantly degrades the image. Changing the smoothly does not appear to help either.
Thanks in advance,
Bryan