Cyclops (24 image stack combined with Helicon Focus)

Optical microscopes + digital photography

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Charles Krebs
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Cyclops (24 image stack combined with Helicon Focus)

Post by Charles Krebs » 06.04.2005 08:26

This is a cyclops photographed on a combound microscope. 24 images were combined to get adequate depth-of-field.
Charles Krebs
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Dan Kozub
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Post by Dan Kozub » 06.04.2005 09:32

this is my favorite! :) just stunning!
Charles, why the legs are "transparent"?
Is there nothing inside of the chitin surface? no muscles?

Charles Krebs
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Post by Charles Krebs » 12.04.2005 00:27

Danylo... there are indeed muscles and other tissue inside the legs, but it just doesn't show in "brightfield" illumination. I guess the refractive index of the tissue is too close to that of water. It becomes more obvious with different types of contrast "enhancing" illumination. It is especially obvious when used crossed polarized lighting. I'll attach a picture of the legs of another micro-creature that was taken using cross polarization and you can see how well the tissue can be seen with this illumination.
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Dan Kozub
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Post by Dan Kozub » 12.04.2005 08:11

Have you tried colored water? :) Just to make muscles visible.

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Juza
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Post by Juza » 24.04.2005 12:09

Fantastic image Charles! 8)
Juza | www.juzaphoto.com

[i]May beauty be everywhere around me[/i]

iamnemo
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Post by iamnemo » 27.12.2005 02:12

Amazing image Charles!

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Ariel
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Post by Ariel » 19.11.2006 06:03

That's so cool!!!

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