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Mosquito pupa (32 image stack using Helicon Focus)

Posted: 06.04.2005 08:22
by Charles Krebs
Image

This is a mosquito pupa photographed on a compound microscope with a low power objective. 32 images were combined to get sufficient depth-of-field.

Charles Krebs

Posted: 27.12.2005 02:21
by iamnemo
Charles, have you thought of printing posters of your pictures? These are classics!

Congratulations again!

Iam Nemo

Mosquito Pupa

Posted: 08.02.2006 19:55
by Bob Town
Hi Charles
My colleagues and I have been admiring your images for some time now, congratulations, we know very well how tricky it can be.
Can you tell me what illumination technique you used to get this superb pupa image, presumably it is a darkfield technique but I suspect there is more to it than that.
Incidentally, in a previous incarnation I was part of the team that invented the original software technology for extended focus (Auto-Montage) and last year we tested Helicon Focus and found it to have the best algorithms compared to several other products.

Re: Mosquito Pupa

Posted: 08.02.2006 23:00
by iamnemo
Hello Bob,

May I ask to what products you compared HeliconFocus to? CombineZ5 for sure but what others? What else is out there?

Does that include Auto-Montage itself? Sorry, could not resist asking :wink: Thanks! Iam
Bob Town wrote:Incidentally, in a previous incarnation I was part of the team that invented the original software technology for extended focus (Auto-Montage) and last year we tested Helicon Focus and found it to have the best algorithms compared to several other products.

Posted: 10.02.2006 06:37
by Charles Krebs
Bob... thanks! This was indeed primarily darkfield (on a compound microscope with a low power objective) but there was a little more to it. I added some illumination from above using fiber optic light guides to get some detail and surface texture in the areas not adequately illuminated by the darkfield lighting.