### Stackshot Movement

Posted:

**02.03.2019 05:13**Hi,

I've been having a bit of trouble finding information on calculating the precise interval for the Stackshot device. I wonder if someone might be able to confirm some things for me. I am using a microscope objective with a Nikon 850 so the usual advice of use the DOF calculator does not apply.

If the Stackshot (by default) moves 1.5875mm per revolution - I am looking for how many 'microsteps' in the HR software to set so that each interval moves the minimum step size for the Stackshot - 1 micron. According to Cognisys - the device moves 3200 microsteps for each 1.5875mm. Does this microstep value equate to the setting in the preferences for 'Size of focusing steps' set to microstep? If so - then theoretically 2 microsteps = 1 micron of movement?

If that is incorrect can someone point me in the right direction? Also - when I set this value I don't hear the Stackshot kick into high precision mode like I should.

Sorry for the rambling....

I've been having a bit of trouble finding information on calculating the precise interval for the Stackshot device. I wonder if someone might be able to confirm some things for me. I am using a microscope objective with a Nikon 850 so the usual advice of use the DOF calculator does not apply.

If the Stackshot (by default) moves 1.5875mm per revolution - I am looking for how many 'microsteps' in the HR software to set so that each interval moves the minimum step size for the Stackshot - 1 micron. According to Cognisys - the device moves 3200 microsteps for each 1.5875mm. Does this microstep value equate to the setting in the preferences for 'Size of focusing steps' set to microstep? If so - then theoretically 2 microsteps = 1 micron of movement?

If that is incorrect can someone point me in the right direction? Also - when I set this value I don't hear the Stackshot kick into high precision mode like I should.

Sorry for the rambling....