Search found 38 matches

by Charles Krebs
12.01.2008 08:49
Forum: Helicon Focus: Macro
Topic: Crab Spider
Replies: 2
Views: 6793

Crab Spider

This is from a stack of 5 images Taken with a Canon 65mm MP-E macro lens on a Canon 5D body. Taken outdoors, natural light, lens was on a Novoflex focusing rail.


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by Charles Krebs
12.01.2008 08:44
Forum: Helicon Focus: Macro
Topic: Blow Fly (Calliphora sp.)
Replies: 4
Views: 10226

Blow Fly (Calliphora sp.)

The stack of images for this fly was taken with a reverse mounted 50mm EL Nikkor on a camera bellows. Camera was a Nikon D200.

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by Charles Krebs
12.01.2008 08:37
Forum: Helicon Focus: Micro
Topic: Water Bear (tardigrade) head
Replies: 0
Views: 9063

Water Bear (tardigrade) head

This is made from a stack on only 5 images. It was taken on a compound microscope using an Olympus 40/0.95 S Plan Apo objective, and DIC (Differential Interference Contrast) illumination. Camera on trinocular tube was a Conon 350D.


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by Charles Krebs
12.01.2008 08:32
Forum: Helicon Focus: Micro
Topic: Lace Bug (Hemiptera)
Replies: 0
Views: 9436

Lace Bug (Hemiptera)

This small bug is fairly common in the moss samples I often examine for micro-critters.

Taken on a compound microscope using a 4/0.16 Olympus S Plan Apo objective. Illuminated from above by fiber optics and small diffusion dome.

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by Charles Krebs
12.01.2008 08:25
Forum: Helicon Focus: Macro
Topic: Midge face
Replies: 2
Views: 5733

Midge face

I had originally ran a stack all the way through this subject, front to back. But it looked to "busy" and confusing so I went back and ran just the frames to get the face, antenna, and legs. This was taken with a 10X Nikon M Plan objective, mounted on the front of a camera bellows, the camera was a ...
by Charles Krebs
26.12.2007 09:34
Forum: Helicon Focus: Support
Topic: How to callibrate scale bar?
Replies: 16
Views: 19264

Danylo, The sensor's physical size, and the pixels per side are specific values that are not prone to error. (Although with some cameras it may take some "Googling" to get the values for the active sensor area). I like the method used by AutoMontage and some of the other "microscope" oriented softwa...
by Charles Krebs
21.12.2007 22:18
Forum: Helicon Focus: Support
Topic: How to callibrate scale bar?
Replies: 16
Views: 19264

Danylo, I forgot to log in when I replied above. But I would like to add some additional thoughts. While I appreciate the ability to place a scale bar in Helicon Focus, I too feel it would be much more useful if it were more "automated". It is a simple calculation, and all that is needed from the us...
by Charles Krebs
03.09.2007 21:38
Forum: Helicon Focus: Macro
Topic: European crane fly, Tipula paludosa
Replies: 4
Views: 9675

European crane fly, Tipula paludosa

Top image taken with Nikon D200, Nikon Epi-5X microscope objective on bellows.

Lower image (eye) was taken with Nikon D200, Nikon 20X U microscope objective on bellows.



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by Charles Krebs
03.09.2007 21:07
Forum: Helicon Focus: Micro
Topic: Moth Eggs
Replies: 2
Views: 9024

Moth Eggs

These small moth eggs were found on a leaf. They are just about ready to hatch. The top image is illuminated from above (using fiber optic illuminators) and you can see the nice texture of the eggs. The second is illuminated from blow (microscope condenser) and you see the small caterpillars inside,...
by Charles Krebs
03.09.2007 20:32
Forum: Helicon Focus: Macro
Topic: Daddy-Longlegs (Harvestman), eyes
Replies: 4
Views: 11620

Daddy-Longlegs (Harvestman), eyes

The eyes of Daddy-Longlegs are fascinating to see. (Again... thanks Danylo for the software that makes shots like this possible!) Nikon D200, Canon 35/2.8 macro bellows lens, on bellows. 81 images in stack. http://www.krebsmicro.com/forumpix/hman1.jpg Nikon D200, Nikon Epi-5X microscope objective on...
by Charles Krebs
10.02.2006 07:47
Forum: Helicon Focus: Macro
Topic: distance of shots?
Replies: 1
Views: 6085

Anne, It depends on the depth-of-field in each picture, which is a function of the aperture (f-stop) used to take the picture. You want to be sure that there is some "overlap" of the "in-focus" part for each image in the series. It is far better to have too many images rather than "out-of-focus" gap...
by Charles Krebs
10.02.2006 06:37
Forum: Helicon Focus: Micro
Topic: Mosquito pupa (32 image stack using Helicon Focus)
Replies: 4
Views: 15365

Bob... thanks! This was indeed primarily darkfield (on a compound microscope with a low power objective) but there was a little more to it. I added some illumination from above using fiber optic light guides to get some detail and surface texture in the areas not adequately illuminated by the darkfi...
by Charles Krebs
10.10.2005 07:40
Forum: Helicon Focus: Micro
Topic: "Sunset Moth" (Urania ripheus), iridescent wing sc
Replies: 1
Views: 12816

"Sunset Moth" (Urania ripheus), iridescent wing sc

This is from a stack of 21 images. It was photographed using a compound microscope with a 10X objective.
by Charles Krebs
10.10.2005 07:37
Forum: Helicon Focus: Micro
Topic: Green metallic Halictid bee
Replies: 10
Views: 22137

Green metallic Halictid bee

This is from a stack of 41 images. Photographed using a compound microscope with a low power objective.
by Charles Krebs
19.04.2005 18:21
Forum: Helicon Focus: Micro
Topic: Fly #2
Replies: 1
Views: 7986

Fly #2

This is a fly photographed from the top. 2.5X objective with 2.5X photo-eyepiece on compound microscope. Approximately 40 images were in the stack. Canon 10D camera, lighting via fiber optic illuminator.