I use the original RAW files (see dropbox link). The rail is only for focussing the first image in minimal focussing distance.
I will try it with less harsh light.
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I download the demo version of Helicon Focus to try the possibilities of the software. I use a tripod, macro rail and the camera internal focus stacking function. The results of all three algorithms was unusble. What did I wrong? You can find the full resolution picture and the 50 RAW images in the ...