Search found 38 matches

by Charles Krebs
28.02.2009 09:56
Forum: Shooting
Topic: lenses - internal focus or not
Replies: 4
Views: 9761

Ian, The program handles the "magnification" changes fine. But that's not the big problem. If the lens entrance pupil moves at all when focus is changed while shooting such a stack (near to far landscape, wide angle lens), then objects (especially those very near the lens, and toward the e...
by Charles Krebs
27.02.2009 20:11
Forum: Helicon Focus: Macro
Topic: Lophacris cristata (grasshopper)
Replies: 1
Views: 4711

Lophacris cristata (grasshopper)

Taken with Nikon D300, 80/4 Componon-S reverse mounted on bellows.


Image
by Charles Krebs
27.02.2009 20:07
Forum: Helicon Focus: Micro
Topic: Chrysochroa fulgens
Replies: 1
Views: 6540

Chrysochroa fulgens

One of the most outrageously iridescent "jewel beetles".
Nikon SMZ-800 stereoscope and the Canon 5D camera.


Image
by Charles Krebs
27.02.2009 20:03
Forum: Helicon Focus: Micro
Topic: Snail eggs
Replies: 0
Views: 6980

Snail eggs

These were taken with a 10X objective using oblique brightfield illumination.
These would move slowly, but occasionally they would all remain still enough to quickly take a stack of images.


Image
by Charles Krebs
04.06.2008 07:24
Forum: Helicon Focus: Macro
Topic: Macro/Landscapes
Replies: 16
Views: 25523

Dan, Yes, naturally there was some touch-up required because of the problem you mention. But not nearly as much as I had feared would be the case. As far as equipment is concerned the most difficult part is getting a wide-angle lens that will allow smooth and continuous focus from over 1:1 out to &q...
by Charles Krebs
04.06.2008 07:06
Forum: Helicon Focus: Micro
Topic: Radiolaria
Replies: 8
Views: 15042

Dan, I can see there is kind of aberration on the images? I think they can be improved by shifting blue channel up and red channel down. What is the reason for this aberration? Not really. These were done with DIC (Differential Interference Contrast) illumination. When used "normally" with...
by Charles Krebs
28.05.2008 02:41
Forum: Helicon Focus: Micro
Topic: Cymbella, Stauroneis... diatoms
Replies: 1
Views: 7030

Cymbella, Stauroneis... diatoms

THe upper image (Cymbella) is from a 6 image stack. The lower image (Stauroneis) is from a 7 image stack. Olympus BHS microscope with DIC illumnation. Canon 350D camera. Diatoms mounted in Zrax. http://www.krebsmicro.com/forumpix/8700-05.jpg http://www.krebsmicro.com/forumpix/8621-27.jpg
by Charles Krebs
28.05.2008 02:31
Forum: Helicon Focus: Micro
Topic: Water Mite (Arrenurus genus)
Replies: 0
Views: 9238

Water Mite (Arrenurus genus)

lluminated with a mix of darkfield (for the legs primarily) and surface illumination via fiber optic light guides.

Olympus BHS microscope, Olympus 4X S Plan Apo objective, Canon 350D. 23 image Z-stack.

Image
by Charles Krebs
28.05.2008 02:21
Forum: Helicon Focus: Micro
Topic: Radiolaria
Replies: 8
Views: 15042

Radiolaria

The fossil skeletons of radiolarians are very three dimensional, making them a tough subject to photograph with a microscope. Stacking with Helicon Focus allows for some nice looks at these structures. Canon 350D above an Olympus BHS microscope. Olympus 20X S Plan Apo objective with DIC illumination...
by Charles Krebs
28.05.2008 02:11
Forum: Helicon Focus: Macro
Topic: Urania riphaeus (Sunset Moth) wing scales
Replies: 0
Views: 4656

Urania riphaeus (Sunset Moth) wing scales

This is from a stack of 19 images. It was taken at 20X (magnification on sensor) using a Canon 40D camera and a Nikon 20/0.40 CF M Plan microscope objective... both mounted onto a Nikon bellows.

Image
by Charles Krebs
28.05.2008 02:07
Forum: Helicon Focus: Macro
Topic: Macro/Landscapes
Replies: 16
Views: 25523

Macro/Landscapes

Here are a couple of "macro/landscapes" made using Helicon Focus. These are from the Quinault Rain Forest in Washington State. The light was very "flat" this day (I would have preferred a brighter overcast), but it was extremely calm which is absolutely essential. You really can'...
by Charles Krebs
01.05.2008 20:50
Forum: Helicon Focus: Micro
Topic: Daphnia (5 image stack)
Replies: 6
Views: 16373

Sorex,
It belongs to the family Daphniidae.
So I think it can be referred to as a daphnia. If not... what is it?
by Charles Krebs
01.05.2008 20:45
Forum: Helicon Focus: Micro
Topic: Centipede head
Replies: 3
Views: 10126

Gene, The resulting image file has the same pixel dimensions as each of source images. For my microscope shots this is 3456x2304 pixels (Canon 350D), for my "macro" shots it is 3872x2592 (Nikon D200). I usually save everything as "tif" files to avoid any compression artifacts. Th...
by Charles Krebs
01.05.2008 20:36
Forum: Helicon Focus: Macro
Topic: Midge face
Replies: 2
Views: 4577

J.D., The 24-105L is a very good lens, but not really a great choice for this kind of magnification. Since you already have extension tubes, a better (but relatively inexpensive) choice would be a reverse mounted enlarging lens or 50mm normal lens (f1.8 or slower). You will also need a way to either...
by Charles Krebs
12.01.2008 09:10
Forum: Helicon Focus: Micro
Topic: Centipede head
Replies: 3
Views: 10126

Centipede head

These are images of fairly small centipedes that were found in moss samples. In both cases looking at the ventral side. The upper image was taken with an Olympus 4/0.16 S Plan Apo, using darkfield illumination. The stack consisted of 19 images. The lower image gives a good look at one of the articul...

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